Lactobacillus pentosus strain LPS16 produces lactic acid, inhibiting multidrug-resistant Helicobacter pylori

doi:10.1016/j.jmii.2014.04.014

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/30938

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30938

Title:	Lactobacillus pentosus strain LPS16 produces lactic acid, inhibiting multidrug-resistant Helicobacter pylori
Authors:	Zheng, Po-Xing Fang, Hsin-Yi Yang, Hsiao-Bai Tien, Nai-Yueh Wang, Ming-Cheng Wu, Jiunn-Jong
Contributors:	Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci Chia Nan Univ Pharm & Sci, Coll Pharm & Sci, Dept Biotechnol Ton Yen Gen Hosp, Dept Pathol Natl Cheng Kung Univ, Coll Med, Clin Pharm & Pharmaceut Sci Natl Cheng Kung Univ Hosp, Dept Internal Med Natl Cheng Kung Univ, Coll Med, Dept Med Lab Sci & Biotechnol Natl Cheng Kung Univ, Ctr Infect Dis & Signal Res
Keywords:	Acid Bactericidal Helicobacter Lactobacillus mice
Date:	2016-04
Issue Date:	2018-01-18 11:38:18 (UTC+8)
Publisher:	Elsevier Taiwan
Abstract:	Background/Purpose: Helicobacter pylori is a human gastric pathogen. Antibiotic resistance of H. pylori has become a problem increasing the failure of H. pylori eradication. Therefore alternative approaches are required. The aim of this study was to evaluate the anti -H. pylori activity of Lactobacillus pentosus strain LPS16 and the mechanism of its killing effect. Methods: The anti -H. pylori activity of LPS16 was determined by the disc diffusion test and time killing assay. High-performance liquid chromatography analysis was used to analyze the secreted compounds of LPS16. Sixty H. pylori strains isolated from different gastric diseases, having different antibiotic susceptibility were collected to analyze the spectrum of anti -H. pylori activity of LPS16. Adhesion ability of LPS16 to gastric epithelial cell lines was assayed by flow cytometry. Results: The anti -H. pylori activity of LPS16 depended on the secreted component, and lactic acid mediated bactericidal activity against H. pylori. The bactericidal activity did not vary significantly among the strains isolated from different diseases having different antibiotic susceptibility. Moreover, LPS16 can adhere on gastric epithelial cell lines AKG and MKN45. Conclusion: L. pentosus strain LPS16 had the broad-spectrum anti -H. pylon activity, suggesting that it can be used to prevent H. pylon infection. Copyright (C) 2014, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC. All rights reserved.
Relation:	Journal of Microbiology Immunology and Infection, v.49 n.2, pp.168-174
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

Files in This Item:

File	Description	Size	Format
30938.pdf		590Kb	Adobe PDF	296	View/Open
index.html		0Kb	HTML	1466	View/Open

Loading...