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https://ir.cnu.edu.tw/handle/310902800/30907
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| 標題: | Reactive Oxygen Species Mediate Terbufos-Induced Apoptosis in Mouse Testicular Cell Lines via the Modulation of Cell Cycle and Pro-Apoptotic Proteins |
| 作者: | Hung, Jui-Hsiang
Chen, Chia-Yun
Omar, Hany A.
Huang, Kuo-Yuan
Tsao, Che-Chia
Chiu, Chien-Chih
Chen, Yi-Ling
Chen, Po-Han
Teng, Yen-Ni |
| 貢獻者: | Chia Nan Univ Pharm & Sci, Dept Biotechnol
Chia Nan Univ Pharm & Sci, Drug Discovery & Dev Ctr
Beni Suef Univ, Fac Pharm, Dept Pharmacol, Bani Suwayf 62514, Egypt
Univ Sharjah, Sharjah Inst Med Res, Dept Pharmacol, Coll Pharm
Natl Cheng Kung Univ, Dept Orthoped, Coll Med
Natl Univ Tainan, Dept Biol Sci & Technol
Kaohsiung Med Univ, Dept Biotechnol
Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
Far East Univ, Dept Cosmet Applicat & Management |
| 關鍵字: | terbufos
caspase-3
reactive oxygen species
rats |
| 日期: | 2016-12 |
| 上傳時間: | 2018-01-18 11:37:41 (UTC+8) |
| 出版者: | Wiley-Blackwell |
| 摘要: | Terbufos (S-t-butylthiomethyl-O, O-diethyl phosphorodithioate) is a highly toxic organophosphate which is extensively used as an insecticide and nematicide. Chronic exposure to terbufos causes neuronal injury and predisposes to neurodegenerative diseases. Accumulating evidence has shown that the exposure to terbufos, as an occupational risk factor, may also cause reproductive disorders. However, the exact mechanisms of reproductive toxicity remain unclear. The present study aimed to investigate the toxic effect of terbufos on testicular cells and to explore the mechanism of toxicity on a cellular level. The cytotoxic effects of terbufos on mouse immortalized spermatogonia (GC-1), spermatocytes (GC-2), Leydig (TM3), and Sertoli (TM4) cell lines were assessed by MTT assays, caspase activation, flow cytometry, TUNEL assay, Western blot, and cell cycle analysis. The exposure to different concentrations of terbufos ranging from 50 to 800 mu M for 6 h caused significant death in all the used testicular cell lines. Terbufos increased reactive oxygen species (ROS) production, reduced mitochondrial membrane potential, and initiated apoptosis, which was confirmed by a dose-dependent increase in the number of TUNEL-positive apoptotic cells. Blocking ROS production by N-acetyl cysteine (NAC) protected GC-1 cells from terbufos-induced cell death. The results demonstrated that terbufos induces ROS, apoptosis, and DNA damage in testicular cell lines and it should be considered potentially hazardous to testis. Together, this study provided potential molecular mechanisms of terbufos-induced toxicity in testicular cells and suggests a possible protective measure. (C) 2015 Wiley Periodicals, Inc. |
| 關聯: | Environmental Toxicology, v.31 n.12, pp.1888-1898 |
| 顯示於類別: | [高齡福祉養生管理系] 期刊論文
[生物科技系(所)] 期刊論文
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