Reactive Oxygen Species Mediate Terbufos-Induced Apoptosis in Mouse Testicular Cell Lines via the Modulation of Cell Cycle and Pro-Apoptotic Proteins

doi:10.1002/tox.22190

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/30907

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30907

Title:	Reactive Oxygen Species Mediate Terbufos-Induced Apoptosis in Mouse Testicular Cell Lines via the Modulation of Cell Cycle and Pro-Apoptotic Proteins
Authors:	Hung, Jui-Hsiang Chen, Chia-Yun Omar, Hany A. Huang, Kuo-Yuan Tsao, Che-Chia Chiu, Chien-Chih Chen, Yi-Ling Chen, Po-Han Teng, Yen-Ni
Contributors:	Chia Nan Univ Pharm & Sci, Dept Biotechnol Chia Nan Univ Pharm & Sci, Drug Discovery & Dev Ctr Beni Suef Univ, Fac Pharm, Dept Pharmacol, Bani Suwayf 62514, Egypt Univ Sharjah, Sharjah Inst Med Res, Dept Pharmacol, Coll Pharm Natl Cheng Kung Univ, Dept Orthoped, Coll Med Natl Univ Tainan, Dept Biol Sci & Technol Kaohsiung Med Univ, Dept Biotechnol Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Far East Univ, Dept Cosmet Applicat & Management
Keywords:	terbufos caspase-3 reactive oxygen species rats
Date:	2016-12
Issue Date:	2018-01-18 11:37:41 (UTC+8)
Publisher:	Wiley-Blackwell
Abstract:	Terbufos (S-t-butylthiomethyl-O, O-diethyl phosphorodithioate) is a highly toxic organophosphate which is extensively used as an insecticide and nematicide. Chronic exposure to terbufos causes neuronal injury and predisposes to neurodegenerative diseases. Accumulating evidence has shown that the exposure to terbufos, as an occupational risk factor, may also cause reproductive disorders. However, the exact mechanisms of reproductive toxicity remain unclear. The present study aimed to investigate the toxic effect of terbufos on testicular cells and to explore the mechanism of toxicity on a cellular level. The cytotoxic effects of terbufos on mouse immortalized spermatogonia (GC-1), spermatocytes (GC-2), Leydig (TM3), and Sertoli (TM4) cell lines were assessed by MTT assays, caspase activation, flow cytometry, TUNEL assay, Western blot, and cell cycle analysis. The exposure to different concentrations of terbufos ranging from 50 to 800 mu M for 6 h caused significant death in all the used testicular cell lines. Terbufos increased reactive oxygen species (ROS) production, reduced mitochondrial membrane potential, and initiated apoptosis, which was confirmed by a dose-dependent increase in the number of TUNEL-positive apoptotic cells. Blocking ROS production by N-acetyl cysteine (NAC) protected GC-1 cells from terbufos-induced cell death. The results demonstrated that terbufos induces ROS, apoptosis, and DNA damage in testicular cell lines and it should be considered potentially hazardous to testis. Together, this study provided potential molecular mechanisms of terbufos-induced toxicity in testicular cells and suggests a possible protective measure. (C) 2015 Wiley Periodicals, Inc.
Relation:	Environmental Toxicology, v.31 n.12, pp.1888-1898
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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