Development of Genetic Dereplication Strains in Aspergillus nidulans Results in the Discovery of Aspercryptin

doi:10.1002/anie.201507097

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標題:	Development of Genetic Dereplication Strains in Aspergillus nidulans Results in the Discovery of Aspercryptin
作者:	Chiang, Yi-Ming Ahuja, Manmeet Oakley, C. Elizabeth Entwistle, Ruth Asokan, Anabanadam Zutz, Christoph Wang, Clay C. C. Oakley, Berl R.
貢獻者:	Univ So Calif, Sch Pharm, Dept Pharmacol & Pharmaceut Sci Chia Nan Univ Pharm & Sci, Dept Pharm Univ Kansas, Dept Mol Biosci Univ Vet Med Vienna, Inst Milk Hyg Univ So Calif, Dept Chem Univ Kansas, Del Shankel Struct Biol Ctr Reliance Ind Ltd, Reliance Technol Grp, Ind Biotechnol Div
關鍵字:	aspergillus biosynthesis genetic dereplication natural products NRPS flavus
日期:	2016-01
上傳時間:	2018-01-18 11:37:40 (UTC+8)
出版者:	Wiley-V C H Verlag Gmbh
摘要:	To reduce the secondary metabolite background in Aspergillus nidulans and minimize the rediscovery of compounds and pathway intermediates, we created a "genetic dereplication" strain in which we deleted eight of the most highly expressed secondary metabolite gene clusters (more than 244,000 base pairs deleted in total). This strain allowed us to discover a novel compound that we designate aspercryptin and to propose a biosynthetic pathway for the compound. Interestingly, aspercryptin is formed from compounds produced by two separate gene clusters, one of which makes the well-known product cichorine. This raises the exciting possibility that fungi use differential regulation of expression of secondary metabolite gene clusters to increase the diversity of metabolites they produce.
關聯:	Angewandte Chemie-International Edition, v.55 n.5, pp.1662-1665
显示于类别:	[藥學系(所)] 期刊論文

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