Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/30906
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30906


    Title: Development of Genetic Dereplication Strains in Aspergillus nidulans Results in the Discovery of Aspercryptin
    Authors: Chiang, Yi-Ming
    Ahuja, Manmeet
    Oakley, C. Elizabeth
    Entwistle, Ruth
    Asokan, Anabanadam
    Zutz, Christoph
    Wang, Clay C. C.
    Oakley, Berl R.
    Contributors: Univ So Calif, Sch Pharm, Dept Pharmacol & Pharmaceut Sci
    Chia Nan Univ Pharm & Sci, Dept Pharm
    Univ Kansas, Dept Mol Biosci
    Univ Vet Med Vienna, Inst Milk Hyg
    Univ So Calif, Dept Chem
    Univ Kansas, Del Shankel Struct Biol Ctr
    Reliance Ind Ltd, Reliance Technol Grp, Ind Biotechnol Div
    Keywords: aspergillus
    biosynthesis
    genetic dereplication
    natural products
    NRPS
    flavus
    Date: 2016-01
    Issue Date: 2018-01-18 11:37:40 (UTC+8)
    Publisher: Wiley-V C H Verlag Gmbh
    Abstract: To reduce the secondary metabolite background in Aspergillus nidulans and minimize the rediscovery of compounds and pathway intermediates, we created a "genetic dereplication" strain in which we deleted eight of the most highly expressed secondary metabolite gene clusters (more than 244,000 base pairs deleted in total). This strain allowed us to discover a novel compound that we designate aspercryptin and to propose a biosynthetic pathway for the compound. Interestingly, aspercryptin is formed from compounds produced by two separate gene clusters, one of which makes the well-known product cichorine. This raises the exciting possibility that fungi use differential regulation of expression of secondary metabolite gene clusters to increase the diversity of metabolites they produce.
    Relation: Angewandte Chemie-International Edition, v.55 n.5, pp.1662-1665
    Appears in Collections:[Dept. of Pharmacy] Periodical Articles

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