Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/30510
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18074/20272 (89%)
Visitors : 4307937      Online Users : 6358
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30510


    Title: Interactions between U-937 human macrophages and tyloxapol
    Authors: Jo-wen Tseng(曾若雯)
    Jung-hua Steven Kuo(郭榮華)
    Contributors: Graduate lnstitute of Pharmaceutical Science, Chia Nan University of Pharmacy and Science
    Date: 2008-07
    Issue Date: 2017-12-04 15:59:12 (UTC+8)
    Abstract: Tyloxapol is reported to prevent macrophages from reacting to endotoxin. However, the intracellular responses that tyloxapol induces in macrophages are still not fully explored. Hence, the objective of this study was to evaluate the intracellular events in macrophages treated with tyloxapol and assess the antioxidant properties of tyloxapolin endotoxin-activated macrophages. Using flow cytometry, we examined intracellular responses in macrophages: reactive oxygen species (ROS) content, mitochondria membrane potential, and cell cycle profiles. We also assessed the antioxidant properties of tyloxapol in endotoxin-activated macrophages. Kinetic hydrogen peroxide production tended to decline with increasing doses. Tyloxapol produced a progressive increase followed by a decline in superoxide anion production in macrophages with increasing doses. Tyloxapol also caused unstable fluctuations in mitochondrial membrane potential. Apoptosis had developed at higher doses after 4h of incubation time. After 2 h of tyloxapol-pretreatment, tyloxapol acted as an antioxidant only at lower doses. Most tyloxapol-pretreated cells at lower doses fully recovered from the changes in superoxide anion and hydrogen peroxide production. Our findings contribute to a better understanding of the molecular action of tyloxapol in macrophages and how it protects macrophages against endotoxin.
    Relation: 第五屆海峽化學、生物及材料研討會，起迄日：2008/07/21-2008/07/22，地點：嘉南藥理科技大學
    Appears in Collections:[Dept. of Pharmacy] Proceedings
    [Pharmacy and Science] The 5rh Conference of Channel-bridge Chemistry, Biology and materal Science

    Files in This Item:

    File Description SizeFormat
    B08.pdf337KbAdobe PDF340View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback