| 摘要: | Less attention was focused on the fungi which were isolated from polluted environment, which might provide a good alternative to search for useful natural products1.Penicillium sp. Strain HLKG-44 is an endophytic fungus isolated from a highly contaminated river in southern China. The extracts of the fungus show high cytotoxicity against sereval human cancer cell lines , such as KB cell (IC50,0.028 g/ml) and Raji cell(IC50, 0.035 g/ml). Little work has been carried out on the fungal genus, Penicillium.During initial investigations into the metabolites of this species, we have isolated three compounds,including a new compound, named as brefeldin A formylate, and two known compounds , brefeldin A and ergosterol. BFA is a macro-cyclic lactone fungal metabolite exhibiting a wide range of antifungal, antiviral, antimitotic, and antitumor activity2 . It has attracted research interest for many years due to its peculiar molecular structure and its bioactivity3,4. Here, we find brefeldin A fromylate for the first time from penicillum sp. strain HLKG-44. Brefeldin A formylate also shows antitumor activity. In the present paper , we report the isolation and structural elucidation of the new compounds 1, together with two known compounds , brefeldin A and ergosterol. The structure of the compound 1 was established by the spectral X-ray crystallographic analysis (Figure 2) |
