| 摘要: | 當歸屬(Angelica L.) 植物在全世界約有 90 種，在台灣目前所栽培的當歸品種，以早年由日本引進的日本當歸種子馴育而來，一般稱之為台灣土當歸 (Angelica acutiloba )。目前對台灣土當歸的研究多停留在其成分分析上，在精油的生物活性評估方面則少有文獻研究，因此本實驗探討台灣土當歸 (Angelica acutiloba )及市售大陸產之川歸(Angelica sinensis)精油進行成分鑑定，並測定其抗菌、抗發炎、抗氧化、美白之能力。
使用水蒸氣蒸餾法 (SD)萃取台灣土當歸全株植物，並利用氣相層析質譜分析儀 (GC-MS)進行成分鑑定，結果顯示 ，台灣土當歸全株精油產率為 4.89%，共鑑定出 33 種化合物，主成分為3-n-butylphthalide (42.87%)、γ-terpinene (24.83%)、β-caryophyllene (8.98%)。市售大陸產之川歸精油成分鑑定，共鑑定出 6 種化合物，主成分為 ethyl linoleate (30.07%)、ethyl oleate (23.43%)、ligustilide (17.80%)。
抗發炎實驗部分，在 NO 清除能力以市售川歸優於 α-bisbolol 標準品，α-bisbolol又優於台灣土當歸；在抑制5-Lox能力測定，α-bisbolol優於市售川歸，市售川歸又優於台灣土當歸。抗氧化實驗部分，在DPPH 自由基清除力以 α-tocopherol 標準品優於市售川歸及台灣土當歸；在 β-胡蘿蔔素-亞麻油酸試驗成效依序為市售川歸、α-tocopherol、台灣土當歸。美白實驗部分，在酪胺酸酶抑制能力，市售川歸及台灣土當歸皆優於 L-ascorbicacid 標準品，兩者比較則以市售川歸較佳。在痤瘡桿菌分泌脂肪酶試驗中，台灣土當歸與市售川歸皆優於標準品α-bisbolol。抗菌實驗部分，S. aureus 、 P. aeruginosa 、 P. acnes 抗菌效果為台灣土當歸較佳，而 E. coli 抗菌效果則為市售川歸較佳。
Angelica acutiloba belongs to the Apiaceae family, which is a perennial herbaceous plant. “Taiwan Angelica acutiloba”,was introduced from Japan. Only few studies have been published on biological activities of essential oil from Angelica acutiloba,therefore ,in our study, we compared the compositions of essential oils between Angelica acutiloba and commercial Angelica sinensis,and their antibacterial,anti-inflammatory , antioxidative,and whitening activities. In this study, we adopted steam-distillation method to extract the essential oil from Taiwan Angelica acutiloba. We used gas chromatography-mass spectrometry (GC-MS) to analyze the components. The yield of essential oil from Angelica acutiloba was 4.89%. The primary components of the essential oil obtained by steam-distillation were 3-n-butylphthalide (42.87%),γ-Terpinene (24.83%) and β-caryophyllene (8.98%). The primary components of the essential oil from Angelica sinensis were ethyl linoleate (30.07%),ethyl oleate (23.43%)and ligustilide (17.80%). In the anti-inflammatory test, the essential oil of Angelica sinensis showed better NO redical scavenging and 5-LOX inhibition than Angelica acutiloba. Both were better P. acnes lipase inhibition than α-bisabolol. In the antioxidant test, α-tocopherol was superior in removing DPPH free radicals to Angelica sinensis and Angelica acutiloba. However the essential oil of Angelica sinensis showed better β-carotene inhibition than Angelica acutiloba. In the whitening activity test, both were better tyrosinase inhibition than L-ascorbicacid. In the antibacterial test, Angelica sinensis had better effects on E. coli than Angelica acutiloba,but on S. aureus, P. aeruginosa and P. acnes Angelica acutiloba showed stronger antibacterial than Angelica sinensis. |
