Multicarotenoids at Physiological Levels Inhibit Metastasis in Human Hepatocarcinoma SK-Hep-1 Cells

doi:10.1080/01635581.2015.1019633

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Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/29740

Title:	Multicarotenoids at Physiological Levels Inhibit Metastasis in Human Hepatocarcinoma SK-Hep-1 Cells
Authors:	Chen, Huei-Yan Yang, Chih-Min Chen, Jen-Yin Yueh, Te-Cheng Hu, Miao-Lin
Contributors:	老人服務事業管理系
Keywords:	Beta-carotene Matrix metalloproteinases Hepatocellular-carcinoma Cancer prevention Alpha-carotene Hepatoma-cells Lycopene Invasion Lutein Expression
Date:	2015-05
Issue Date:	2016-04-19 19:06:29 (UTC+8)
Publisher:	Routledge Journals
Abstract:	Several studies have demonstrated that single carotenoid, including lycopene, beta-carotene, and alpha-carotene, exhibits antimetastatic effects; however, little is known whether multicarotenoids have similar effects. Herein, we investigated the antimetastatic effect of multicarotenoids at physiological serum levels in Taiwanese (MCT at 1.4 mu M) and American (MCA at 1.8 mu M) populations using human hepatocarcinoma SK-Hep-1 cells in comparison with single carotenoid, such as lycopene (0.3 or 0.6 mu M, respectively), alpha-carotene (0.1 mu M), beta-carotene (0.4 mu M), lutein (0.4 or 0.5 mu M, respectively), and beta-cryptoxanthin (0.2 mu M). Results reveal that MCA treatment exhibited an additive inhibition on invasion, migration and adhesion at 24 and 48h of incubation, whereas MCT treatment possessed additive inhibition at 48h of incubation. The antimetastatic action of MCT and MCA involved additive reduction on activities of matrix metalloproteinase (MMP)-2, -9, and protein expression of Rho and Rac 1 but additive promotion on protein expression of tissue inhibitor of MMP (TIMP)-1 and -2. All of these effects were stronger in MCA than in MCT at 24 and 48h of incubation. These results demonstrate that multi-carotenoids effectively inhibit metastasis of human hepatocarcinoma SK-Hep-1 cells. More in vivo studies are needed to confirm these findings.
Relation:	Nutrition And Cancer-An International Journal, v.67 n.4, pp.676-686
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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