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https://ir.cnu.edu.tw/handle/310902800/29657
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| 標題: | Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes |
| 作者: | Lee, Chen-Hsuan
Yang, Yu-Min
Leu, Kuen-Lin
Lin, Hung-Yin
Liang, Chia-Hua
Chang, Chien-Hsiang |
| 貢獻者: | 化粧品應用與管理系 |
| 關鍵字: | Catanionic vesicles
Catanionic vesicle/DNA complex
Ion pair amphiphile
Vesicle/DNA association
Vesicular bilayer |
| 日期: | 2015-08 |
| 上傳時間: | 2016-04-19 19:03:33 (UTC+8) |
| 出版者: | Springer |
| 摘要: | Cationic vesicles fabricated from various novel materials have been recently developed for DNA delivery applications, and the physical stability of the vesicle/DNA complexes played an important role in the transfection efficiency. In this study, positively charged catanionic vesicles composed of hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), ditetradecyldimethylammonium bromide (DTDAB), and cholesterol were prepared in buffer solution by a forced formation approach, and the association behavior of the catanionic vesicles with two types of DNA molecules with the consideration of the vesicle/DNA complex stability was explored by dynamic light scattering, fluorescence polarization, and DNA electrophoresis techniques. In a buffer solution of pH 7.4, HTMA-DS/DTDAB/cholesterol catanionic vesicles with high physical stability and positive charge character were successfully fabricated. A toxicity examination then implied that the catanionic vesicles have the potential of being applied in transfection applications. It is noted that the catanionic vesicle dispersions became unstable as prepared from a concentrated one by dilution due to the less charged vesicle surfaces and the more rigid vesicular bilayers, which could be explained by the enhanced dissolution of HTMA(+) from the vesicles. The physical stability and charge character of the catanionic vesicle/DNA complexes were found strongly dependent on the mixing ratio of the vesicle to DNA. The formation of the catanionic vesicle/DNA complexes was also confirmed by an electrophoresis analysis. Moreover, not only the electrostatic repulsion between the vesicle/DNA complexes but also the molecular packing of the vesicular bilayers played roles in governing the physical stability of the vesicle/DNA complexes. |
| 關聯: | Colloid And Polymer Science, v.293 n.8, pp.2239-2247 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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