Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes

doi:10.1007/s00396-015-3608-6

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 化妝品應用與管理系(所) > 期刊論文 > Item 310902800/29657

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標題:	Exploring physical stability characteristics of positively charged catanionic vesicle/DNA complexes
作者:	Lee, Chen-Hsuan Yang, Yu-Min Leu, Kuen-Lin Lin, Hung-Yin Liang, Chia-Hua Chang, Chien-Hsiang
貢獻者:	化粧品應用與管理系
關鍵字:	Catanionic vesicles Catanionic vesicle/DNA complex Ion pair amphiphile Vesicle/DNA association Vesicular bilayer
日期:	2015-08
上傳時間:	2016-04-19 19:03:33 (UTC+8)
出版者:	Springer
摘要:	Cationic vesicles fabricated from various novel materials have been recently developed for DNA delivery applications, and the physical stability of the vesicle/DNA complexes played an important role in the transfection efficiency. In this study, positively charged catanionic vesicles composed of hexadecyltrimethylammonium-dodecylsulfate (HTMA-DS), ditetradecyldimethylammonium bromide (DTDAB), and cholesterol were prepared in buffer solution by a forced formation approach, and the association behavior of the catanionic vesicles with two types of DNA molecules with the consideration of the vesicle/DNA complex stability was explored by dynamic light scattering, fluorescence polarization, and DNA electrophoresis techniques. In a buffer solution of pH 7.4, HTMA-DS/DTDAB/cholesterol catanionic vesicles with high physical stability and positive charge character were successfully fabricated. A toxicity examination then implied that the catanionic vesicles have the potential of being applied in transfection applications. It is noted that the catanionic vesicle dispersions became unstable as prepared from a concentrated one by dilution due to the less charged vesicle surfaces and the more rigid vesicular bilayers, which could be explained by the enhanced dissolution of HTMA(+) from the vesicles. The physical stability and charge character of the catanionic vesicle/DNA complexes were found strongly dependent on the mixing ratio of the vesicle to DNA. The formation of the catanionic vesicle/DNA complexes was also confirmed by an electrophoresis analysis. Moreover, not only the electrostatic repulsion between the vesicle/DNA complexes but also the molecular packing of the vesicular bilayers played roles in governing the physical stability of the vesicle/DNA complexes.
關聯:	Colloid And Polymer Science, v.293 n.8, pp.2239-2247
显示于类别:	[化妝品應用與管理系(所)] 期刊論文

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