In Vitro Activity of Imipenem and Colistin against a Carbapenem-Resistant Klebsiella pneumoniae Isolate Coproducing SHV-31, CMY-2, and DHA-1

doi:10.1155/2015/568079

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/29578

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/29578

Title:	In Vitro Activity of Imipenem and Colistin against a Carbapenem-Resistant Klebsiella pneumoniae Isolate Coproducing SHV-31, CMY-2, and DHA-1
Authors:	Tang, Hung-Jen Ku, Yee-Huang Lee, Mei-Feng Chuang, Yin-Ching Yu, Wen-Liang
Contributors:	保健營養系
Keywords:	Spectrum beta-lactamases Enterobacteriaceae Combination Infections Therapy TAIWAN
Date:	2015
Issue Date:	2016-04-19 19:00:53 (UTC+8)
Publisher:	Hindawi Publishing Corporation
Abstract:	We investigated the synergism of colistin and imipenem against a multidrug-resistant K. pneumoniae isolate which was recovered from a severe hip infection. PCR and DNA sequencing were used to characterize the outermembrane porin genes and the resistance genes mediating the common beta-lactamases and carbapenemases. Synergism was evaluated by time-kill studies. The bla(SHV-31), bla(CMY-2), and bla(DHA-1) were detected. Outer membrane porin genes analysis revealed loss of ompK36 and frame-shift mutation of ompK35. The common carbapenemase genes were not found. Time-kill studies demonstrated that a combination of 1x MIC of colistin (2 mg/L) and 1x MIC of imipenem (8 mg/L) was synergistic and bactericidal but with inoculum effect. Bactericidal activity without inoculum effect was observed by concentration of 2x MIC of colistin alone or plus 2x MIC of imipenem. In conclusion, colistin plus imipenem could be an alternative option to treat carbapenem-resistant K. pneumoniae infections.
Relation:	Biomed Research International,V.2015 ,Article ID 568079
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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