Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography

doi:10.1155/2015/450547

CNU IR > College of Sustainable Environment and Recreation > Dept. of Environmental Engineering and Science (including master's program) > Periodical Articles > Item 310902800/29576

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/29576

Title:	Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography
Authors:	Wei, Ming-Chi Yang, Yu-Chiao Hong, Show-Jen
Contributors:	環境工程與科學系
Keywords:	Barbata D.don Rp-HPLC separation Iridoid glucosides CO2 extraction Herbal tea Optimization Lycopene Solubility Globulus Seeds
Date:	2015
Issue Date:	2016-04-19 19:00:49 (UTC+8)
Publisher:	Hindawi Publishing Corporation
Abstract:	Oleanolic acid (OA) and ursolic acid (UA) were extracted from Hedyotis diffusa using a hyphenated procedure of ultrasound-assisted and supercritical carbon dioxide (HSC-CO2) extraction at different temperatures, pressures, cosolvent percentages, and SC-CO2 flow rates. The results indicated that these parameters significantly affected the extraction yield. The maximal yields of OA (0.917 mg/g of dry plant) and UA (3.540 mg/g of dry plant) were obtained at a dynamic extraction time of 110 min, a static extraction time of 15 min, 28.2 MPa, and 56 degrees C with a 12.5% (v/v) cosolvent (ethanol/water = 82/18, v/v) and SC-CO2 flowing at 2.3 mL/min (STP). The extracted yields were then analyzed by high performance liquid chromatography (HPLC) to quantify the OA and UA. The present findings revealed that H. diffusa is a potential source of OA and UA. In addition, using the hyphenated procedure for extraction is a promising and alternative process for recovering OA and UA from H. diffusa at high concentrations.
Relation:	Evidence-Based Complementary And Alternative Medicine, Article ID 450547
Appears in Collections:	[Dept. of Environmental Engineering and Science (including master's program)] Periodical Articles

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