| 標題: | Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases |
| 作者: | Liu, Ting
Sanchez, James F.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C. |
| 貢獻者: | 化妝品應用與管理系 |
| 關鍵字: | ASPERGILLUS-NIDULANS
BIOSYNTHETIC-PATHWAY
CRYSTAL-STRUCTURE
METABOLITE
SYSTEM |
| 日期: | 2014-03 |
| 上傳時間: | 2015-05-06 21:24:19 (UTC+8) |
| 出版者: | Amer Chemical Soc |
| 摘要: | A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly created hybrid with the NR-PKS AN3386 is able to accept a longer starter unit in place of the native substrate to create a novel aromatic polyketide in vivo. |
| 關聯: | Organic Letters, v.16 n.6, pp.1676-1679 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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