Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/28605

請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/28605

標題:	Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin
作者:	Yiu, Ching-Yi Chen, Shih-Ying Yang, Tsai-Hsiu Chang, Che-Jung Yeh, Dong-Bor Chen, Yi-Jie Lin, Tsuey-Pin
貢獻者:	保健營養系生物科技系
關鍵字:	Polygonum cuspidatum emodin antiviral activity EBV
日期:	2014-01
上傳時間:	2015-05-06 21:22:15 (UTC+8)
出版者:	Mdpi Ag
摘要:	Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
關聯:	Molecules, v.19 n.1, pp.1258-1272
顯示於類別:	[保健營養系(所) ] 期刊論文 [生物科技系(所)] 期刊論文

文件中的檔案:

檔案	描述	大小	格式	瀏覽次數
index.html		0Kb	HTML	1632	檢視/開啟

在CNU IR中所有的資料項目都受到原著作權保護.

TAIR相關文章