Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

doi:10.3390/molecules19011258

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Title:	Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin
Authors:	Yiu, Ching-Yi Chen, Shih-Ying Yang, Tsai-Hsiu Chang, Che-Jung Yeh, Dong-Bor Chen, Yi-Jie Lin, Tsuey-Pin
Contributors:	保健營養系生物科技系
Keywords:	Polygonum cuspidatum emodin antiviral activity EBV
Date:	2014-01
Issue Date:	2015-05-06 21:22:15 (UTC+8)
Publisher:	Mdpi Ag
Abstract:	Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
Relation:	Molecules, v.19 n.1, pp.1258-1272
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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