English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17744/20032 (89%)
Visitors : 7256614      Online Users : 312
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/28605


    標題: Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin
    作者: Yiu, Ching-Yi
    Chen, Shih-Ying
    Yang, Tsai-Hsiu
    Chang, Che-Jung
    Yeh, Dong-Bor
    Chen, Yi-Jie
    Lin, Tsuey-Pin
    貢獻者: 保健營養系
    生物科技系
    關鍵字: Polygonum cuspidatum
    emodin
    antiviral activity
    EBV
    日期: 2014-01
    上傳時間: 2015-05-06 21:22:15 (UTC+8)
    出版者: Mdpi Ag
    摘要: Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.
    關聯: Molecules, v.19 n.1, pp.1258-1272
    Appears in Collections:[保健營養系(所) ] 期刊論文
    [生物科技系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML466View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback