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| 標題: | Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin |
| 作者: | Yiu, Ching-Yi
Chen, Shih-Ying
Yang, Tsai-Hsiu
Chang, Che-Jung
Yeh, Dong-Bor
Chen, Yi-Jie
Lin, Tsuey-Pin |
| 貢獻者: | 保健營養系
生物科技系 |
| 關鍵字: | Polygonum cuspidatum
emodin
antiviral activity
EBV |
| 日期: | 2014-01 |
| 上傳時間: | 2015-05-06 21:22:15 (UTC+8) |
| 出版者: | Mdpi Ag |
| 摘要: | Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug. |
| 關聯: | Molecules, v.19 n.1, pp.1258-1272 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
[生物科技系(所)] 期刊論文
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