Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.

doi:10.1155/2014/953873

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/28504

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/28504

Title:	Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.
Authors:	Wang, Lisu Chiou, Shu-Yuan Shen, Yi-Ting Yen, Fu-Tsun Ding, Hsiou-Yu Wu, Ming-Jiuan
Contributors:	藥學系生物科技系化粧品應用與管理系
Keywords:	MACROPHAGE INFLAMMATORY PROTEIN-1 HEME OXYGENASE-1 NITRIC-OXIDE RAW264.7 MACROPHAGES MOUSE MACROPHAGES TNF-ALPHA KAPPA-B LIPOPOLYSACCHARIDE INHIBITION EXPRESSION
Date:	2014
Issue Date:	2015-05-06 21:18:39 (UTC+8)
Publisher:	Hindawi Publishing Corporation
Abstract:	The green fruit of Solanum integrifolium Poir. has been used traditionally as an anti-inflammatory and analgesic remedy in Taiwanese aboriginal medicine. The goal of this study is to evaluate the anti-inflammatory activity and mechanism of the green fruit extract of S. integrifolium. A bioactivity-guided fractionation procedure was developed to identify the active partition fraction. The methanol fraction (ME), with the highest phenolic content, exhibited the strongest inhibitory effect against LPS-mediated nitric oxide (NO) release and cytotoxicity in RAW264.7 macrophages. ME also significantly downregulated the expression of LPS-induced proinflammatory genes, such as iNOS, COX-2, IL-1 beta, IL-6, CCL2/MCP-1, and CCL3/MIP1 alpha. Moreover, ME significantly upregulated HO-1 expression and stimulated the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2). Pretreatment of cells with the HO-1 inhibitor zinc protoporphyrin and MEK/ERK inhibitor U0126 attenuated ME's inhibitory activity against LPS-induced NO production. Taken together, this is the first study to demonstrate the anti-inflammatory activity of green fruit extract of S. integrifolium and its activity may be mediated by the upregulation of HO-1 expression and activation of ERK1/2 pathway.
Relation:	Biomed Research International, 953873
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Pharmacy] Periodical Articles

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