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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/28403

    標題: 水溶性煤焦色素之檢出與定量-DEAE-Sephadex柱層層析法之應用
    Analysis of Water Soluble Coal-Tar Dyes with DEAE-Sephadex Column Chromatogra phy
    作者: 陳憲弘
    貢獻者: 私立嘉南藥學專科學校
    日期: 1976
    上傳時間: 2014-12-25 15:02:33 (UTC+8)
    摘要: 本實驗是利用DEAE-Sephadex將含於飲食食物煤焦色素(coal-tar dyes)吸附於Column之頂層後進行柱層層析法以分離之。最初以含3%NH4CI及40%EtOH水億溶出,然後將NH4CI濃度逐漸增加至溶出液100ml時為15%。將分離之色素進一步根據顏色,溶出體積(Elution volum;Ve),吸附光譜及於λmax處之吸光值以達到定性及定量之目的。
    The DEAE-Sephadex was used in this experiment to absorb and concen-trate the permitted coal-tar dyes in foods on the top of the column. Separation of the coacentrated coal-tar dyes was performed by elution with 40% EtOH solution coataining 3% NH4CI at the begining and gradual increasing of the NH4CI concentration to 15% as elution volume reached 1OOml. Seperated coal-tar dyes were offered for qualitative and quatitative analyses according to their color, elution volume, absorption spectra and absorbance at λmax
    關聯: 嘉南學報, n.2 pp.17-28
    Appears in Collections:[ 食品科技系 ] 期刊論文
    [嘉南學報] 2期 (1976)

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