本實驗是利用DEAE-Sephadex將含於飲食食物煤焦色素(coal-tar dyes)吸附於Column之頂層後進行柱層層析法以分離之。最初以含3%NH4CI及40%EtOH水億溶出，然後將NH4CI濃度逐漸增加至溶出液100ml時為15%。將分離之色素進一步根據顏色，溶出體積(Elution volum；Ve)，吸附光譜及於λmax處之吸光值以達到定性及定量之目的。 The DEAE-Sephadex was used in this experiment to absorb and concen-trate the permitted coal-tar dyes in foods on the top of the column. Separation of the coacentrated coal-tar dyes was performed by elution with 40% EtOH solution coataining 3% NH4CI at the begining and gradual increasing of the NH4CI concentration to 15% as elution volume reached 1OOml. Seperated coal-tar dyes were offered for qualitative and quatitative analyses according to their color, elution volume, absorption spectra and absorbance at λmax
