本研究在移動相中添加5 mM β-環糊精於甲醇與水的混合溶液，利用RP-C18 靜相管柱分離一些單取代酚類位置異構物，並探討移動相中甲醇含量的影響。由層析結果顯示甲醇與水比例，會顯著影響單取代酚?位置?構物的分?效果。添加 5 mM β-環糊精於適當比例的甲醇與水的混合溶液當移動相，利用RP-C18 靜相管柱可以有效分離硝基酚、甲酚、乙酚以及萘酚位置異構物，且硝基酚與乙酚可以達到基線分離。 The retention behavior of some isomeric mono-substituted phenols was investigated by high-performance liquid chromatography on a RP-C18 stationary phase using β-cyclodextrin as mobile phase additives. Effects of methanol content of mobile phase were examined. Effective separations of some isomeric mono-substituted phenols were achieved on a RP-C18 stationary phase using β-cyclodextrin as mobile phase additives. Moreover, baseline separations of isomeric nitrophenol and ethylphenol were achieved.
