Triptolide Ameliorates Autoimmune Diabetes and Prolongs Islet Graft Survival in Nonobese Diabetic Mice

doi:10.1097/MPA.0b013e318269d076

CNU IR > College of Sustainable Environment and Recreation > Dept. of Recreation and Health-Care Management > Periodical Articles > Item 310902800/27954

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27954

Title:	Triptolide Ameliorates Autoimmune Diabetes and Prolongs Islet Graft Survival in Nonobese Diabetic Mice
Authors:	Huang, Shing-Hwa Lin, Gu-Jiun Chu, Chi-Hong Yu, Jyh-Cherng Chen, Teng-Wei Chen, Yuan-Wu Chien, Ming-Wei Chu, Chin-Chen Sytwu, Huey-Kang
Contributors:	休閒保健管理系
Keywords:	Triptolide Type 1 Diabetes Islet Transplantation Nod Mice Autoimmune Recurrence Allograft Rejection
Date:	2013-04
Issue Date:	2014-05-26 10:50:28 (UTC+8)
Publisher:	Lippincott Williams & Wilkins
Abstract:	Objectives: Triptolide (TPL) possesses profound immunosuppressive effects and has potential in allograft transplantation. We investigated whether TPL treatment prevents autoimmune diabetes in nonobese diabetic (NOD) mice and prolongs the survival of islet grafts against autoimmune attack or allograft rejection.Methods: Diabetic incidence was monitored in TPL-treated NOD mice. Nonobese diabetic or BALB/c islets were transplanted into diabetic recipients treated with TPL. Different T-cell subsets in grafts or spleen were analyzed. The proliferation, apoptosis, cytokines, and activities of AKT, NF kappa B, and caspases 3, 8, and 9 of T cells were determined.Results: Diabetic incidence was reduced and inflammatory cytokines were decreased in islets and spleen under TPL treatment. T-cell proliferation was reduced and the survival of syngeneic or allogeneic grafts was significantly increased in TPL-treated mice. The populations of CD4, CD8, CD4CD69, CD8CD69, and interferon-gamma-producing T cells in islet grafts and spleen were reduced. Triptolide treatment increased the apoptosis of T cells in the spleen of recipients. Levels of phosphorylated protein kinase B and phosphorylated inhibitor of kappa B in splenocytes were reduced and caspases 3, 8, and 9 were increased in TPL-treated mice.Conclusions: Triptolide treatment not only reduced the diabetic incidence in NOD mice but also prolonged the survival of syngeneic or allogeneic grafts.
Relation:	Pancreas, v.42 n.3, pp.442-451
Appears in Collections:	[Dept. of Recreation and Health-Care Management] Periodical Articles

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