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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27840

    標題: Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
    作者: Yeh, Hsu-Hua
    Chang, Shu-Lin
    Chiang, Yi-Ming
    Bruno, Kenneth S.
    Oakley, Berl R.
    Wu, Tung-Kung
    Wang, Clay C. C.
    貢獻者: 藥學系
    關鍵字: Aspergillus-Nidulans
    日期: 2013-02
    上傳時間: 2014-05-26 10:45:48 (UTC+8)
    出版者: Amer Chemical Soc
    摘要: We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA In A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA Delta R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
    關聯: Organic Letters, v.15 n.4 pp.756-759
    Appears in Collections:[藥學系(所)] 期刊論文
    [生物科技系(所)] 期刊論文

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