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https://ir.cnu.edu.tw/handle/310902800/27840
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| Title: | Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping |
| Authors: | Yeh, Hsu-Hua
Chang, Shu-Lin
Chiang, Yi-Ming
Bruno, Kenneth S.
Oakley, Berl R.
Wu, Tung-Kung
Wang, Clay C. C. |
| Contributors: | 藥學系
生物科技系 |
| Keywords: | Aspergillus-Nidulans
Genes
Biosynthesis
Identification
Cyclization
Release
System |
| Date: | 2013-02 |
| Issue Date: | 2014-05-26 10:45:48 (UTC+8) |
| Publisher: | Amer Chemical Soc |
| Abstract: | We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA In A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA Delta R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes. |
| Relation: | Organic Letters, v.15 n.4 pp.756-759 |
| Appears in Collections: | [Dept. of Pharmacy] Periodical Articles
[Dept. of Biotechnology (including master's program)] Periodical Articles
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