Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

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Title:	Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
Authors:	Yeh, Hsu-Hua Chang, Shu-Lin Chiang, Yi-Ming Bruno, Kenneth S. Oakley, Berl R. Wu, Tung-Kung Wang, Clay C. C.
Contributors:	藥學系生物科技系
Keywords:	Aspergillus-Nidulans Genes Biosynthesis Identification Cyclization Release System
Date:	2013-02
Issue Date:	2014-05-26 10:45:48 (UTC+8)
Publisher:	Amer Chemical Soc
Abstract:	We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA In A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbA Delta R+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
Relation:	Organic Letters, v.15 n.4 pp.756-759
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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