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| 標題: | Effects of citrus flavonoids, 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone and 3,5,6,7,8,3 ',4 '-heptamethoxyflavone, on the activities of macrophage scavenger receptors and the hepatic LDL receptor |
| 作者: | Kou, Mei-Chun
Fu, Shih-Hang
Yen, Jui-Hung
Weng, Ching-Yi
Li, Shiming
Ho, Chi-Tang
Wu, Ming-Jiuan |
| 貢獻者: | 生物科技系 |
| 關鍵字: | Low-Density-Lipoprotein
Foam Cell-Formation
Monocyte-Derived Macrophages
Ester-Induced Expression
Gene-Expression
Thp-1 Cells
Alpha-Tocopherol
Cd36 Expression
Increases Cd36
In-Vitro |
| 日期: | 2013-04 |
| 上傳時間: | 2014-05-26 10:45:34 (UTC+8) |
| 出版者: | Royal Soc Chemistry |
| 摘要: | Epidemiological and animal studies point to a possible protective effect of citrus flavonoids against cardiovascular diseases. The aim of this study is to investigate the effects of citrus flavonoids, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF) and 3,5,6,7,8,3',4'-heptamethoxyflavone (HpMF), on the activities and expressions of macrophage scavenger receptors and the hepatic LDL receptor. Treatment of HpMF (20 mu M) during THP-1 differentiation successfully attenuated 12-myristate 13-acetate (PMA)-mediated DiI-labeled oxidized low-density lipoprotein (oxLDL) uptake as evidenced by flow cytometry, indicating that the functions of scavenger receptors were blocked. RT-Q-PCR analysis suggests that the decrease in oxLDL uptake was due to the down-regulation of PMA-induced SR-A mRNA expression. In terminally differentiated THP-1 macrophages, 5-OH-HxMF and HpMF could significantly reduce DiI-oxLDL uptake, with the former having a greater effect. 5-OH-HxMF attenuated oxLDL-mediated CD36 and SR-A expression; while HpMF only decreased CD36 expression. The effects of these two flavonoids on the activity and expression of the hepatic LDL receptor (LDLR) were further investigated in HepG2 cells. 5-OH-HxMF (10-20 mu M) enhanced DiI-LDL uptake by 1.33-fold due to the enhanced LDLR expression. These results imply that HpMF is better at inhibiting PMA-induced oxLDL uptake during THP1 differentiation, while 5-OH-HxMF is more powerful in attenuating oxLDL-induced scavenger receptor expression and activity in terminally differentiated THP-1 macrophages. Furthermore, 5-OH-HxMF may have hypolipidemic activity due to its up-regulating hepatic LDLR expression. |
| 關聯: | Food & Function, v.4 n.4 pp.602-609 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
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