Effects of citrus flavonoids, 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone and 3,5,6,7,8,3 ',4 '-heptamethoxyflavone, on the activities of macrophage scavenger receptors and the hepatic LDL receptor

doi:10.1039/c3fo30301b

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/27834

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27834

Title:	Effects of citrus flavonoids, 5-hydroxy-3,6,7,8,3 ',4 '-hexamethoxyflavone and 3,5,6,7,8,3 ',4 '-heptamethoxyflavone, on the activities of macrophage scavenger receptors and the hepatic LDL receptor
Authors:	Kou, Mei-Chun Fu, Shih-Hang Yen, Jui-Hung Weng, Ching-Yi Li, Shiming Ho, Chi-Tang Wu, Ming-Jiuan
Contributors:	生物科技系
Keywords:	Low-Density-Lipoprotein Foam Cell-Formation Monocyte-Derived Macrophages Ester-Induced Expression Gene-Expression Thp-1 Cells Alpha-Tocopherol Cd36 Expression Increases Cd36 In-Vitro
Date:	2013-04
Issue Date:	2014-05-26 10:45:34 (UTC+8)
Publisher:	Royal Soc Chemistry
Abstract:	Epidemiological and animal studies point to a possible protective effect of citrus flavonoids against cardiovascular diseases. The aim of this study is to investigate the effects of citrus flavonoids, 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF) and 3,5,6,7,8,3',4'-heptamethoxyflavone (HpMF), on the activities and expressions of macrophage scavenger receptors and the hepatic LDL receptor. Treatment of HpMF (20 mu M) during THP-1 differentiation successfully attenuated 12-myristate 13-acetate (PMA)-mediated DiI-labeled oxidized low-density lipoprotein (oxLDL) uptake as evidenced by flow cytometry, indicating that the functions of scavenger receptors were blocked. RT-Q-PCR analysis suggests that the decrease in oxLDL uptake was due to the down-regulation of PMA-induced SR-A mRNA expression. In terminally differentiated THP-1 macrophages, 5-OH-HxMF and HpMF could significantly reduce DiI-oxLDL uptake, with the former having a greater effect. 5-OH-HxMF attenuated oxLDL-mediated CD36 and SR-A expression; while HpMF only decreased CD36 expression. The effects of these two flavonoids on the activity and expression of the hepatic LDL receptor (LDLR) were further investigated in HepG2 cells. 5-OH-HxMF (10-20 mu M) enhanced DiI-LDL uptake by 1.33-fold due to the enhanced LDLR expression. These results imply that HpMF is better at inhibiting PMA-induced oxLDL uptake during THP1 differentiation, while 5-OH-HxMF is more powerful in attenuating oxLDL-induced scavenger receptor expression and activity in terminally differentiated THP-1 macrophages. Furthermore, 5-OH-HxMF may have hypolipidemic activity due to its up-regulating hepatic LDLR expression.
Relation:	Food & Function, v.4 n.4 pp.602-609
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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