Development of a hyphenated procedure of heat-reflux and ultrasound-assisted extraction followed by RP-HPLC separation for the determination of three flavonoids content in Scutellaria barbata D. Don

doi:10.1016/j.jchromb.2013.09.015

Chia Nan University of Pharmacy & Science Institutional Repository > 環境永續暨休閒學院 > 環境工程與科學系(所) > 期刊論文 > Item 310902800/27824

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標題:	Development of a hyphenated procedure of heat-reflux and ultrasound-assisted extraction followed by RP-HPLC separation for the determination of three flavonoids content in Scutellaria barbata D. Don
作者:	Wei, Ming-Chi Yang, Yu-Chiao Chiu, Hui-Fen Hong, Show-Jen
貢獻者:	環境工程與科學系
關鍵字:	Scutellaria Barbata D. Don Ultrasound-Assisted Extraction Hplc Apigenin Baicalin Luteolin
日期:	2013-12
上傳時間:	2014-05-26 10:45:09 (UTC+8)
出版者:	Elsevier Science Bv
摘要:	A hyphenated procedure of heat-reflux and ultrasound-assisted extraction (HUAE), and an accurate high-performance liquid chromatographic (HPLC) method were developed for the determination of apigenin, baicalin and luteolin content in Scutellaria barbara D. Don. The suitable HUAE conditions for the extraction of target compounds from the herb were identified as an ultrasonic frequency of 40 kHz, power of 185 W, duty cycle of 75% (intermittent sonication), mean particle size of 0.355 mm, extraction temperature of 50 degrees C, ratio of solvent to raw material of 12:1 (mL/g), ethanol concentration of 60% (v/v), extraction time of 30 min and three cycles. Compared with a traditional heat-reflux extraction method, the proposed method reduced the extraction time, extraction temperature and solvent consumption. Also, this HUAE method achieved superior apigenin, baicalin and luteolin yields. Furthermore, the developed HUAE-HPLC method was applied successfully for the simultaneous evaluation of three bioactive compounds in five samples of S. barbara D. Don obtained from different geographical regions. These results clearly demonstrated that the combined HUAE-HPLC process is feasible in the future commercialized manufacture of this highly valuable Chinese herbal medicine. (C) 2013 Elsevier B.V. All rights reserved.
關聯:	Journal of Chromatography B-Analytical Technologies In the Biomedical And Life Sciences, v.940 pp.126-134
顯示於類別:	[環境工程與科學系(所)] 期刊論文

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