Development of a hyphenated procedure of heat-reflux and ultrasound-assisted extraction followed by RP-HPLC separation for the determination of three flavonoids content in Scutellaria barbata D. Don

doi:10.1016/j.jchromb.2013.09.015

CNU IR > College of Sustainable Environment and Recreation > Dept. of Environmental Engineering and Science (including master's program) > Periodical Articles > Item 310902800/27824

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Title:	Development of a hyphenated procedure of heat-reflux and ultrasound-assisted extraction followed by RP-HPLC separation for the determination of three flavonoids content in Scutellaria barbata D. Don
Authors:	Wei, Ming-Chi Yang, Yu-Chiao Chiu, Hui-Fen Hong, Show-Jen
Contributors:	環境工程與科學系
Keywords:	Scutellaria Barbata D. Don Ultrasound-Assisted Extraction Hplc Apigenin Baicalin Luteolin
Date:	2013-12
Issue Date:	2014-05-26 10:45:09 (UTC+8)
Publisher:	Elsevier Science Bv
Abstract:	A hyphenated procedure of heat-reflux and ultrasound-assisted extraction (HUAE), and an accurate high-performance liquid chromatographic (HPLC) method were developed for the determination of apigenin, baicalin and luteolin content in Scutellaria barbara D. Don. The suitable HUAE conditions for the extraction of target compounds from the herb were identified as an ultrasonic frequency of 40 kHz, power of 185 W, duty cycle of 75% (intermittent sonication), mean particle size of 0.355 mm, extraction temperature of 50 degrees C, ratio of solvent to raw material of 12:1 (mL/g), ethanol concentration of 60% (v/v), extraction time of 30 min and three cycles. Compared with a traditional heat-reflux extraction method, the proposed method reduced the extraction time, extraction temperature and solvent consumption. Also, this HUAE method achieved superior apigenin, baicalin and luteolin yields. Furthermore, the developed HUAE-HPLC method was applied successfully for the simultaneous evaluation of three bioactive compounds in five samples of S. barbara D. Don obtained from different geographical regions. These results clearly demonstrated that the combined HUAE-HPLC process is feasible in the future commercialized manufacture of this highly valuable Chinese herbal medicine. (C) 2013 Elsevier B.V. All rights reserved.
Relation:	Journal of Chromatography B-Analytical Technologies In the Biomedical And Life Sciences, v.940 pp.126-134
Appears in Collections:	[Dept. of Environmental Engineering and Science (including master's program)] Periodical Articles

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