The protective effects of water extracts of sugarcane (Saccharum officinarum L.) leaves among three varieties, including 28NG256, wild type, and ROC10, on t-BHP-induced oxidative stress in Clone 9 cells were systematically compared. Among these three varieties, 28NG256 showed the highest protective effect against 0.2 mM t-BHP-induced oxidative stress in Clone 9 cells. In addition, 28NG256 displayed higher inhibitory effects on ROS generation than wild type and ROC10. Moreover, 28NG256 showed higher positive regulated GSH levels and antioxidant enzymes as well as higher protective potential against cell death by inhibiting caspase-3 activity and mitochondrial membrane depolarization. Chlorogenic and caffeic acids present in 28NG256 decreased significantly the generation of ROS, which may partly be responsible for the effect of Clone 9 cell growth. Thus, 28NG256, among the three varieties studied, showed the most protective effects against t-BHP-induced oxidative stress in Clone 9 cells. (C) 2013 Elsevier Ltd. All rights reserved.