Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti

doi:10.1016/j.pestbp.2013.08.004

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標題:	Biochemical and molecular analyses to determine pyrethroid resistance in Aedes aegypti
作者:	Lin, Ying-Hsi Tsen, Wei-Lun Tien, Nai-Yueh Luo, Yi-Pey
貢獻者:	生物科技系
關鍵字:	Aedes Aegypti Permethrin Resistance Knockdown Resistant. Standard Glass Cylinder Method Detoxification Enzyme Activity Taqman (R) Probe Assay
日期:	2013-10
上傳時間:	2014-05-26 10:43:32 (UTC+8)
出版者:	Academic Press Inc Elsevier Science
摘要:	Dengue fever is an important mosquito-borne viral disease in Taiwan. Insecticide resistance has been shown to significantly reduce the efficacy of vector control interventions. The detection of insecticide resistance is an important component in mosquito abatement programs. In this study, we used the insecticide-impregnated papers bioassay method to reveal high levels of resistance to permethrin in the LYPR and field strains of Aedes aegypti. We used the standard glass cylinder method to observe the knockdown effect of paralysis within 2 to 4 minutes after exposing mosquitoes to pyrethroid vapors. Biochemical assays showed elevated detoxification enzyme activities. Glutathione S-transferases, monooxygenases and beta-esterases were the enzymes predominantly responsible for the permethrin resistance of Ae. aegypti in Taiwan. Molecular screening for common insecticide target-site mutations revealed the presence of V1023G and D1794Y mutations. Pearson's correlation analysis was used to investigate the correlations between the allelic frequency of kdr mutation associated increase with the LC50 values of permethrin and the KT50 values of pyrethroid vaporizers. These findings will be used to assess resistance levels, estimate resistance potential, and formulate monitoring and resistance management strategies. (C) 2013 Elsevier Inc. All rights reserved.
關聯:	Pesticide Biochemistry And Physiology v.107 n.2 pp.266-276
顯示於類別:	[生物科技系(所)] 期刊論文

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