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| 標題: | Preventive Effects of beta-Thujaplicin Against UVB-Induced MMP-1 and MMP-3 mRNA Expressions in Skin Fibroblasts |
| 作者: | Cherng, Jong Yuh
Chen, Li Yin
Shih, Mei Fen |
| 貢獻者: | 藥學系 |
| 關鍵字: | Uvb
Beta-Thujaplicin
Mmp-1
Mmp-3
Procollagen Gene
Skin Fibroblast |
| 日期: | 2012 |
| 上傳時間: | 2014-03-24 15:22:31 (UTC+8) |
| 出版者: | World Scientific Publ Co Pte Ltd |
| 摘要: | Solar UV radiation damages human skin by affecting skin tone and resiliency and leads to premature aging (photoaging). The skin damage is caused by the activation of the AP-1 transcription factor, which increases matrix metalloproteinase (MMP) expression and collagen degradation. An increase of interleukin (IL)-6 is also correlated with the activation of MMP-1 expression. beta-thujaplicin has shown both acaricidal and antimicrobial activities. Also, beta-thujaplicin has been shown to be protective against apoptosis due to the oxidative effects of UV irradiation. However, the effect of beta-thujaplicin on UVB-induced MMPs had not been investigated. In this study, after UVB exposure, MMP-1 and IL-6 production in human skin fibroblasts was examined in the presence of beta-thujaplicin, vitamin C, and vitamin E. The expression of MMP-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP-1, TIMP-3) and procollagen mRNA was also investigated. Results showed that UVB-induced MMP-1 production was suppressed by the beta-thujaplicin treatment in a dose-dependent manner, but not by vitamin C and vitamin E. beta-thujaplicin also prevented the up-regulation of MMP-1 and MMP-3 mRNA. Moreover, the UVB-suppressed procollagen gene expression was restored to normal by beta-thujaplicin. Neither UVB nor beta-thujaplicin affected the mRNA expression of TIMP-1 and TIMP-3. The IL-6 production induced by UVB was lower in beta-thujaplicin treated fibroblasts than in the controls. In conclusion, this study shows the capability of beta-thujaplicin in preventing MMP-1 production due to UVB irradiation via inhibition of MMP gene expression. Importantly, the UVB-suppressed procollagen gene expression can be restored to normal by beta-thujaplicin. These findings indicate that beta-thujaplicin is a promising and potent agent to inhibit UVB-induced MMP-1 and MMP-3 gene expression in skin fibroblasts. |
| 關聯: | American Journal of Chinese Medicine, 40(2), 387-398 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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