Preventive Effects of beta-Thujaplicin Against UVB-Induced MMP-1 and MMP-3 mRNA Expressions in Skin Fibroblasts

doi:10.1142/S0192415X12500309

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 藥學系(所) > 期刊論文 > Item 310902800/27715

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標題:	Preventive Effects of beta-Thujaplicin Against UVB-Induced MMP-1 and MMP-3 mRNA Expressions in Skin Fibroblasts
作者:	Cherng, Jong Yuh Chen, Li Yin Shih, Mei Fen
貢獻者:	藥學系
關鍵字:	Uvb Beta-Thujaplicin Mmp-1 Mmp-3 Procollagen Gene Skin Fibroblast
日期:	2012
上傳時間:	2014-03-24 15:22:31 (UTC+8)
出版者:	World Scientific Publ Co Pte Ltd
摘要:	Solar UV radiation damages human skin by affecting skin tone and resiliency and leads to premature aging (photoaging). The skin damage is caused by the activation of the AP-1 transcription factor, which increases matrix metalloproteinase (MMP) expression and collagen degradation. An increase of interleukin (IL)-6 is also correlated with the activation of MMP-1 expression. beta-thujaplicin has shown both acaricidal and antimicrobial activities. Also, beta-thujaplicin has been shown to be protective against apoptosis due to the oxidative effects of UV irradiation. However, the effect of beta-thujaplicin on UVB-induced MMPs had not been investigated. In this study, after UVB exposure, MMP-1 and IL-6 production in human skin fibroblasts was examined in the presence of beta-thujaplicin, vitamin C, and vitamin E. The expression of MMP-1, MMP-3, tissue inhibitor of metalloproteinase (TIMP-1, TIMP-3) and procollagen mRNA was also investigated. Results showed that UVB-induced MMP-1 production was suppressed by the beta-thujaplicin treatment in a dose-dependent manner, but not by vitamin C and vitamin E. beta-thujaplicin also prevented the up-regulation of MMP-1 and MMP-3 mRNA. Moreover, the UVB-suppressed procollagen gene expression was restored to normal by beta-thujaplicin. Neither UVB nor beta-thujaplicin affected the mRNA expression of TIMP-1 and TIMP-3. The IL-6 production induced by UVB was lower in beta-thujaplicin treated fibroblasts than in the controls. In conclusion, this study shows the capability of beta-thujaplicin in preventing MMP-1 production due to UVB irradiation via inhibition of MMP gene expression. Importantly, the UVB-suppressed procollagen gene expression can be restored to normal by beta-thujaplicin. These findings indicate that beta-thujaplicin is a promising and potent agent to inhibit UVB-induced MMP-1 and MMP-3 gene expression in skin fibroblasts.
關聯:	American Journal of Chinese Medicine, 40(2), 387-398
显示于类别:	[藥學系(所)] 期刊論文

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