English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17760/20057 (89%)
Visitors : 7977636      Online Users : 249
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27676

    標題: Detection of Copper Ions in Liquid Foods and Beverages Based on an Enzymatic Method
    作者: Yang, Jing-Iong
    Yeh, Dong-Bor
    Kuo, Jen-Min
    Pan, Bonnie Sun
    Lee, Guo-Chi
    Liu, Ya-Hui
    Lai, Ying-Jang
    貢獻者: 生物科技系
    關鍵字: Enzymatic Method
    Superoxide Dismutase
    Copper Ion
    Elsia Microplate Reader
    日期: 2012-03
    上傳時間: 2014-03-21 16:17:18 (UTC+8)
    出版者: Bureau Food Drug Analysis
    摘要: An economic, simple and sensitive enzymatic method for the determination of copper in aqueous samples was proposed in this study. By removal of copper, superoxide dismutase (SOD) becomes apoenzyme SOD (apo-SOD) that has no SOD activity. The amount of copper added can be estimated by the recovery of SOD activity from reconstituted apo-SOD. The concentration of copper in an aqueous sample can thus be determined. A linear relationship (R-2 = 0.998) between the concentrations of copper, ranging from 20 to 80 ppb, and the recovered SOD activities was observed. The detection limit for copper in the proposed assay was 20 ppb. Moreover, interfering effects of some common metals on the recovery of SOD activity was evaluated. It showed that the concentrations of these metals less than 45 mu g/mL exhibited no interference in the assay. In addition, data obtained from this assay were very close to those estimated by inductively-coupled plasma atomic emission spectrometry (ICP-AES). This enzymatic method can be adapted as a routine assay for the determination of copper in aqueous samples such as beverages, drinking water and liquid foods. The assay can be applied for large number of samples (about 96 samples per hour) on an ELISA microplate reader.
    關聯: Journal of Food And Drug Analysis, v.20n.1 pp.83-87
    Appears in Collections:[生物科技系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback