Detection of Copper Ions in Liquid Foods and Beverages Based on an Enzymatic Method

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標題:	Detection of Copper Ions in Liquid Foods and Beverages Based on an Enzymatic Method
作者:	Yang, Jing-Iong Yeh, Dong-Bor Kuo, Jen-Min Pan, Bonnie Sun Lee, Guo-Chi Liu, Ya-Hui Lai, Ying-Jang
貢獻者:	生物科技系
關鍵字:	Enzymatic Method Superoxide Dismutase Apoenzyme Copper Ion Elsia Microplate Reader
日期:	2012-03
上傳時間:	2014-03-21 16:17:18 (UTC+8)
出版者:	Bureau Food Drug Analysis
摘要:	An economic, simple and sensitive enzymatic method for the determination of copper in aqueous samples was proposed in this study. By removal of copper, superoxide dismutase (SOD) becomes apoenzyme SOD (apo-SOD) that has no SOD activity. The amount of copper added can be estimated by the recovery of SOD activity from reconstituted apo-SOD. The concentration of copper in an aqueous sample can thus be determined. A linear relationship (R-2 = 0.998) between the concentrations of copper, ranging from 20 to 80 ppb, and the recovered SOD activities was observed. The detection limit for copper in the proposed assay was 20 ppb. Moreover, interfering effects of some common metals on the recovery of SOD activity was evaluated. It showed that the concentrations of these metals less than 45 mu g/mL exhibited no interference in the assay. In addition, data obtained from this assay were very close to those estimated by inductively-coupled plasma atomic emission spectrometry (ICP-AES). This enzymatic method can be adapted as a routine assay for the determination of copper in aqueous samples such as beverages, drinking water and liquid foods. The assay can be applied for large number of samples (about 96 samples per hour) on an ELISA microplate reader.
關聯:	Journal of Food And Drug Analysis, v.20n.1 pp.83-87
显示于类别:	[生物科技系(所)] 期刊論文

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