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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/27671

    標題: Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol in Aspergillus nidulans
    作者: Lo, Hsien-Chun
    Entwistle, Ruth
    Guo, Chun-Jun
    Ahuja, Manmeet
    Szewczyk, Edyta
    Hung, Jui-Hsiang
    Chiang, Yi-Ming
    Oakley, Berl R.
    Wang, Clay C.C.
    貢獻者: 生物科技系
    關鍵字: Melia-Azedarach
    Penicillium Sp
    Efflux Pump
    日期: 2012-03-14
    上傳時間: 2014-03-21 16:17:08 (UTC+8)
    出版者: Amer Chemical Soc
    摘要: Meroterpenoids are a class of fungal natural products that are produced from polyketide and terpenoid precursors. An understanding of meroterpenoid biosynthesis at the genetic level should facilitate engineering of second-generation molecules and increasing production of first-generation compounds. The filamentous fungus Aspergillus nidulans has previously been found to produce two meroterpenoids, austinol and dehydroaustinol. Using targeted deletions that we created, we have determined that, surprisingly, two separate gene clusters are required for meroterpenoid biosynthesis. One is a cluster of four genes including a polyketide synthase gene, ausA. The second is a cluster of 10 additional genes including a prenyltransferase gene, ausN, located on a separate chromosome. Chemical analysis of mutant extracts enabled us to isolate 3,5-dimethylorsellinic acid and 10 additional meroterpenoids that are either intermediates or shunt products from the biosynthetic pathway. Six of them were identified as novel meroterpenoids in this study. Our data, in aggregate, allow us to propose a complete biosynthetic pathway for the A. nidulans meroterpenoids.
    關聯: Journal of The American Chemical Society, 134(10), 4709-4720
    Appears in Collections:[生物科技系(所)] 期刊論文
    [藥學系(所)] 期刊論文

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