Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol in Aspergillus nidulans

doi:10.1021/ja209809t

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/27671

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27671

Title:	Two Separate Gene Clusters Encode the Biosynthetic Pathway for the Meroterpenoids Austinol and Dehydroaustinol in Aspergillus nidulans
Authors:	Lo, Hsien-Chun Entwistle, Ruth Guo, Chun-Jun Ahuja, Manmeet Szewczyk, Edyta Hung, Jui-Hsiang Chiang, Yi-Ming Oakley, Berl R. Wang, Clay C.C.
Contributors:	生物科技系藥學系
Keywords:	Melia-Azedarach Penicillium Sp Efflux Pump Polyketide Transporter Metabolites Fungus Ustus Identification Cercosporin
Date:	2012-03-14
Issue Date:	2014-03-21 16:17:08 (UTC+8)
Publisher:	Amer Chemical Soc
Abstract:	Meroterpenoids are a class of fungal natural products that are produced from polyketide and terpenoid precursors. An understanding of meroterpenoid biosynthesis at the genetic level should facilitate engineering of second-generation molecules and increasing production of first-generation compounds. The filamentous fungus Aspergillus nidulans has previously been found to produce two meroterpenoids, austinol and dehydroaustinol. Using targeted deletions that we created, we have determined that, surprisingly, two separate gene clusters are required for meroterpenoid biosynthesis. One is a cluster of four genes including a polyketide synthase gene, ausA. The second is a cluster of 10 additional genes including a prenyltransferase gene, ausN, located on a separate chromosome. Chemical analysis of mutant extracts enabled us to isolate 3,5-dimethylorsellinic acid and 10 additional meroterpenoids that are either intermediates or shunt products from the biosynthetic pathway. Six of them were identified as novel meroterpenoids in this study. Our data, in aggregate, allow us to propose a complete biosynthetic pathway for the A. nidulans meroterpenoids.
Relation:	Journal of The American Chemical Society, 134(10), 4709-4720
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Pharmacy] Periodical Articles

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