Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/26941
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 18055/20253 (89%)
造访人次 : 25165265      在线人数 : 524
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    標題: Apoptotic Effect of Cordycepin and Cisplatin on OC3 Human Oral Cancer Cells
    作者: Chen, Ying-hui
    Hao, Lyh-Jyh
    Hung, Chih-peng
    Chen, Jung-wei
    Leu, Sew-fen
    Huang, Bu-miin
    貢獻者: 溫泉產業研究所
    日期: 2011-06
    上傳時間: 2013-09-28 16:25:06 (UTC+8)
    出版者: Springer-Verlag
    摘要: Objective
    To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells.

    The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions.

    Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P<0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P<0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P<0.05).

    Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
    關聯: Chinese Journal of Integrative Medicine, Vol.20 No.8, pp.624-632
    显示于类别:[觀光事業管理系(含溫泉所)] 期刊論文


    档案 描述 大小格式浏览次数

    在CNU IR中所有的数据项都受到原著作权保护.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈