Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/26941
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    標題: Apoptotic Effect of Cordycepin and Cisplatin on OC3 Human Oral Cancer Cells
    作者: Chen, Ying-hui
    Hao, Lyh-Jyh
    Hung, Chih-peng
    Chen, Jung-wei
    Leu, Sew-fen
    Huang, Bu-miin
    貢獻者: 溫泉產業研究所
    日期: 2011-06
    上傳時間: 2013-09-28 16:25:06 (UTC+8)
    出版者: Springer-Verlag
    摘要: Objective
    To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells.

    Methods
    The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions.

    Results
    Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P<0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P<0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P<0.05).

    Conclusion
    Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
    關聯: Chinese Journal of Integrative Medicine, Vol.20 No.8, pp.624-632
    显示于类别:[觀光事業管理系(含溫泉所)] 期刊論文

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