Apoptotic Effect of Cordycepin and Cisplatin on OC3 Human Oral Cancer Cells

doi:10.1007/s11655-013-1453-3

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Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/26941

Title:	Apoptotic Effect of Cordycepin and Cisplatin on OC3 Human Oral Cancer Cells
Authors:	Chen, Ying-hui Hao, Lyh-Jyh Hung, Chih-peng Chen, Jung-wei Leu, Sew-fen Huang, Bu-miin
Contributors:	溫泉產業研究所
Date:	2011-06
Issue Date:	2013-09-28 16:25:06 (UTC+8)
Publisher:	Springer-Verlag
Abstract:	Objective To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods The influences of cisplatin (2.5 μg/mL) and/or cordycepin treatment (10 or 100 μmol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results Data demonstrated that co-administration of cisplatin (2.5 μg/mL) and cordycepin (10 or 100 μmol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P<0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P<0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P<0.05). Conclusion Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
Relation:	Chinese Journal of Integrative Medicine, Vol.20 No.8, pp.624-632
Appears in Collections:	[Dept. of Tourism Management] Periodical Articles

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