本研究在移動相中添加HP-β-CD，利用RP-C18 靜相以分離6 種二氯酚異構物。結果顯示在移動相中，HP-β-環糊精的濃度及甲醇與水比例，會顯著影響二氯酚異構物的分離效果。經由層析圖得知，添加1.25 mM HP- β-環糊精於甲醇與水比例為50：50 的混合溶液當移動相，利用RP-C18 靜相管柱可以有效分離6種二氯酚異構物。從二氯酚異構物的滯留行為可知，包藏錯合物在分離機轉上扮演重要的角色。 The isomeric dichlorophenols were separated by high-performance liquid chromatography using HP-β-cyclodextrin as mobile phase additives. Effects of HP-β-CD concentration and methanol content of mobile phase on the separation of isomeric dichlorophenols were investigated. Chromatogram of six isomeric dichlorophenols shows that the effective separations were achieved on a RPC18 stationary phase using 1.25mM HP-β-cyclodextrin as mobile phase additives. The chromatographic results indicate that inclusion complexes play a dominant role in the retention mechanism.
