移動相中添加HP-β-環糊精在RPLC上分離二氯酚異構物

CNU IR > Chna Nan Annual Bulletin > Vol.38 (2012) > Item 310902800/26836

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Title:	移動相中添加HP-β-環糊精在RPLC上分離二氯酚異構物 Separation of Isomeric Dichlorophenols by RPLC Using HP-β-cyclodextrin as Mobile Phase Additives
Authors:	廖麗芳楊朝成黃嘉慧張書文林振興
Contributors:	輔英科技大學應用化學及材料科學系嘉南藥理科技大學化粧品科技研究所
Keywords:	逆相液相層析 HP-β-環糊精二氯酚 Reversed-Phase Liquid Chromatography (RPLC) HP-β-cyclodextrin dichlorophenol
Date:	2012-12
Issue Date:	2013-08-14 10:42:22 (UTC+8)
Abstract:	本研究在移動相中添加HP-β-CD，利用RP-C18 靜相以分離6 種二氯酚異構物。結果顯示在移動相中，HP-β-環糊精的濃度及甲醇與水比例，會顯著影響二氯酚異構物的分離效果。經由層析圖得知，添加1.25 mM HP- β-環糊精於甲醇與水比例為50：50 的混合溶液當移動相，利用RP-C18 靜相管柱可以有效分離6種二氯酚異構物。從二氯酚異構物的滯留行為可知，包藏錯合物在分離機轉上扮演重要的角色。 The isomeric dichlorophenols were separated by high-performance liquid chromatography using HP-β-cyclodextrin as mobile phase additives. Effects of HP-β-CD concentration and methanol content of mobile phase on the separation of isomeric dichlorophenols were investigated. Chromatogram of six isomeric dichlorophenols shows that the effective separations were achieved on a RPC18 stationary phase using 1.25mM HP-β-cyclodextrin as mobile phase additives. The chromatographic results indicate that inclusion complexes play a dominant role in the retention mechanism.
Relation:	嘉南學報(科技類) 38期：p.55-60
Appears in Collections:	[Chna Nan Annual Bulletin] Vol.38 (2012) [Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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