| 標題: | Melanogenesis inhibition by a crude extract of Magnolia officinalis |
| 作者: | Ding, Hsiou-Yu
Chang, Te-Sheng
Chiang, Chien-Min
Li, Sin-Yi
Tseng, Deng-Yu |
| 貢獻者: | 化粧品科技研究所
生物科技系(所)
1996-0875 |
| 關鍵字: | Magnolia officinalis
melanogenesis
tyrosinase
melanin
inhibition |
| 日期: | 2011-01 |
| 上傳時間: | 2013-06-03 15:52:49 (UTC+8) |
| 出版者: | Academic Journals |
| 摘要: | In the present study, we investigated the inhibitory effect of a crude extract from Magnolia officinalis (MOE) on melanogenesis in both mouse B16 melanoma cells and zebrafish. Our results showed that MOE inhibited melanogenesis in either a-melanocyte stimulating hormone (a-MSH)- or 3-isobutyl-1-methylxanthin (IBMX)-stimulated B16 cells in a dose-dependent manner with an IC50 value of 9.3 µg/ml. In addition, MOE also inhibited cellular tyrosinase activity with an IC50 value of 13.4 µg/ml while no inhibitory activity was found by MOE against cell-free tyrosinase activity. Moreover, western blotting and real time reverse-transcription polymerase chain reaction (qRT-PCR) analyses, respectively confirmed that MOE downregulated levels of tyrosinase protein but not that of its mRNA in a-MSH-stimulated B16 cells. These results demonstrated that MOE inhibits melanogenesis of B16 cells by a pre-translational regulation on tyrosinase gene expression. On the other hand, when using zebrafish as a depigmenting assay system, MOE could inhibit both melanogenesis and tyrosinase activity in the in vivo model. From the present study, MOE was proven to be a good candidate as a skin-whitening agent for treatment of skin hyperpigmentation. |
| 關聯: | Journal of Medicinal Plants Research 5(2), pp. 237-244 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
[生物科技系(所)] 期刊論文
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