Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/260
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/260


    Title: 發展並應用高特異性alcohol dehydrogenase gene (type III) primer快速檢測醋酸菌
    Authors: 王淑珍
    Contributors: 食品科技系
    Date: 2006
    Issue Date: 2008-05-14 16:26:33 (UTC+8)
    Publisher: 台南縣：嘉南藥理科技大學食品科技系
    Abstract: 本研究利用已發表之AAB (acetic acid bacteria) type III adh sequence 設計 primer ADH III L187 / ADH III R500與AAB之reference strains進行PCR反應，可產生 313 bp的PCR產物。其他如Bacillus cereus， Yersinia enterocolitica、Salmonella enterica subsp.、Lactobacillus amylovorus、Pseudomonas aeruginosa及E.coli等非醋酸菌之菌株，則無任何 PCR 產物生成。此外，本實驗共篩選出75 株疑似醋酸菌，經 ADH III L187 / ADH III R500 增幅後，可產生313 bp PCR產物有 41 株，進一步應用Trcek et al.發表之alcohol dehydrogenase gene ( type I ) primer檢測AAB。結果顯示所分析之AAB菌株皆為 Acetobacter spp.，由此顯示 ADH III L187 / ADH III R500 primer對於醋酸菌具有高特異性。
    Relation: 計畫編號 : CNFS9504
    Appears in Collections:[Dept. of Food Science & Technology] Chna Project

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