| 摘要: | 植物精油帶有特殊芳香氣味，文獻指出薄荷具抗氧化、抗發炎、及抗微生物的效用。綠薄荷Spearmint（Mentha spicata）、奧地利薄荷Austrian mint (Mentha × gracilis)、萊姆薄荷Lime mint （Mentha aquatica）為多年生草本植物，夏季耐熱，適合生長於台灣等亞熱帶區域，氣味芳香溫和，廣泛作為香料、香精，用於食品、化粧品中。因此，本研究擬分析並比較綠薄荷、奧地利薄荷與萊姆薄荷精油之成分，並評估其抗氧化、抗發炎與抗菌活性。
首先，以水蒸氣法萃取薄荷精油，再利用氣相層析質譜法(GC-MS)進行成分分析。結果顯示，綠薄荷精油主成分為D(+)-Carvone (52.0%)、D-Limonene (17.2%)、Dihydrocarvone (4.9%)；奧地利薄荷精油主成分為D(+)-Carvone(47.3%)、β-Caryophyllene (7.1%)、D-Limonene (4.6%)，二者均以酮類的含量最高；而萊姆薄荷精油主成分為D-Limonene (41.0%)、D(+)-Carvone (8.5 %)、δ-Selinene (6.7 %)，以萜烯類為最高。雖然其主要成分相似，但成分比例上確實有差異。抗菌評估部分，整體上綠薄荷對於E. coli、S. aureus、P. aeruginosa、C. albicans、P. acnes和 P. ovale抗菌效果優於奧地利薄荷與萊姆薄荷；抗氧化活性評估則顯示，包括DPPH自由基清除力、β-胡蘿蔔素-亞麻油酸試驗均是以奧地利薄荷精油最佳，優於綠薄荷與萊姆薄荷；在NO自由基清除能力與抗發炎部分萊姆薄荷優於奧地利薄荷與綠薄荷。
Plant Essential oils be provided with a special fragrance. The literature indicated that the mint had antioxidant, anti-inflammatory and antibacterial effects.Spearmint, austrian mint, lime mint are perennial herbaceous plants, which are suitable for growth in Taiwan and other sub-tropical regions. Furthermore, they adapt to the climate in Taiwan, and with high heat-resistant in summer. Because their fragrances are mild, they are used wildly as flavors, fragrances, foods, and cosmetics. This study intends to analyze and compare the ingredients of spearmint, austrian mint, and lime mint essential oils and to evaluate their antioxidant, anti-inflammatory and antibacterial activities.Firstly, we extracted the mint essential oils by steam distillation extraction, then analyze the composition by gaschromatography-mass spectrometry (GC-MS).
The results showed that the components of spearmint oil is D(+)-Carvone (52.0%)、D-Limonene (17.2%)、Dihydrocarvone (4.9%).
The components of austrian mint essential oil are D(+)-Carvone(47.3%)、β-Caryophyllene (7.1%)、D-Limonene (4.6%).Ketones have the highest concentrations for both of them. The main component of lime mint essential oil are D-Limonene (41.0%)、D(+)-Carvone (8.5 %)、δ-Selinene (6.7 %).
Terpenes have the highest concentrations for lime mint.Although their main compositions are similar, but indeedly there are difference in the proporation of ingredients.
In the anti-bacterial evaluation, the anti-bacterial effects of spearmint against E. coli ,S. aureus ,P. aeruginosa ,C. albicans ,P. acnes and P. ovale are better than austrian mint and lime mint.The anti-oxidative activity assessment shows that the austrian mint essential oil is the best one in DPPH scavenging radical activity, in β-Carovtene-linoleic acid assay,and better than the spearmint and lime mint. However, the lime mint is better than austrian mint and spearmint in NO scavenging radical activity and anti-inflammatory . |
