Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/25388
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17776/20117 (88%)
Visitors : 10945160      Online Users : 290
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item:

    Title: Simultaneous analysis of nine components in patch preparations of Ru-Yi-Jin-Huang San by high-performance liquid chromatography
    Authors: Horng-Liang Lay
    Chia-Chi Chen
    Shiow-Chyn Huang
    Thau-Ming Cham
    Tian-Shung Wu
    I-Hsin Lin
    Contributors: 藥物科技研究所
    Keywords: Ru-Yi-Jin-Huang-San
    Emodin - Sennoside A - Sennoside B
    Date: 2010-04
    Issue Date: 2012-06-04 16:40:15 (UTC+8)
    Abstract: A facile high-performance liquid chromatography (HPLC) method for the resolution and quantitative measurement of nine marker substances, the active ingredients in patch preparations of Ru-Yi-Jin-Huang-San, was established using gradient elution in the reversed-phase mode. These marker substances included berberine (Phellodendri Cortex), curcumin (Curcumae Rhizoma), imperatorin (Angelicae Dahuricae Radix), magnolol (Magnoliae Cortex), hesperidin (Citri Leiocarpae Exocarpium), glycyrrhizin (Glycyrrhizae Radix), and emodin, sennoside A, sennoside B (Rhei Rhizoma). The ingredients in the water-based and oil-based patches of the formula from different manufactures were also analyzed for quality evaluation. Extracted samples were analyzed by HPLC using a reversed-phase column (Inertsil 5 ODS-2, 4.6-mm I.D. ×250 mm) at 30°C and eluted with a mixture of 20 and 70% acetonitrile aqueous solution in gradient manner at a flow rate of 1.0 ml/min. The detection wavelength varied with time as follows: 275 nm, 0–72 min; 250 nm, 72–105 min; 220 nm, 105–145 min. Relative coefficients of variations of intra- and interday analysis were less than 5%. All the recoveries were 93.30–113.63%. This method could be applied for the simultaneous determination of nine marker substances in Ru-Yi-Jin-Huang-San.
    Relation: Journal of Natural Medicine 64(2):p.194-202
    Appears in Collections:[Dept. of Pharmacy] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    99_160_j.pdf402KbAdobe PDF449View/Open

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback