Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/24736
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 18076/20274 (89%)
造访人次 : 4614284      在线人数 : 1180
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/24736


    標題: Necrotic cell death induced by betanodavirus requires viral RNA replication-dependent that mediated via viral death inducers
    作者: Hung-Cheng Wu
    Jen-Leih Wu
    Heuy-Ling Chu
    Jiann-Ruey Hong
    貢獻者: 食品科技系
    日期: 2009-07-18
    上傳時間: 2011-11-09 14:46:46 (UTC+8)
    摘要: The RNA nervous necrosis virus induces necrotic cell death in fish, but its molecular mechanism is still unsolved. In this study, we demonstrated that betanodavirus-induced mitochondria-mediated necrotic cell death is initiated at the level of RNA replication and requires key viral death inducers at the middle-late replication stage. We determined that viral genomic replication is required for red-spotted grouper nervous necrosis virus (RGNNV)-induced cell death. Results indicated that UV irradiation of the virus could effectively block viral replication and cell death. We cloned the RGNNV RNA dependent RNA polymerase (RdRp; termed protein A) and determined if it was involved in the induction of cell death. Results indicated that protein A was initially expressed 48 h post-infection and localized to the cytoplasm. Furthermore, knockdown of protein A expression completely block viral genomic replication and blocked expression of the viral death inducer proteins α and B2, which correlated with inhibition of phosphatidylserine exposure, mitochondria-mediated death signaling, and an increased cell viability at 72 h post-infection. Furthermore, the knockdown of protein g expression by siRNA, partially inhibited necrotic cell death following RGNNV infection. Together, these results indicated that RGNNV-induced mitochondria-mediated necrotic cell death is dependent on viral RNA replication-dependent viral death inducers and their expression at the middle-late replication stage. These findings may provide new insights into RNA virus-induced host pathogenesis.
    關聯: 2009第二屆抗病毒大會(World summit of antivirals)，起迄日：2009/7/18~2009/7/20，地點：北京
    显示于类别:[ 食品科技系 ] 會議論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    98_101_pro.pdf103KbAdobe PDF1054检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈