English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17744/20032 (89%)
Visitors : 7230875      Online Users : 326
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/24628

    標題: 新穎生物可降解性之奈米型藥物載體PEG-dsDNA合成及細胞內釋控之研究
    Studies on Syntheses of Novel Biodegradable Nanoscale Drug Carriers PEG-dsDNA and Intracellular Drug Delivery
    作者: 楊釗凱
    貢獻者: 嘉南藥理科技大學:藥物科技研究所
    關鍵字: 聚乙二醇
    Drug vector
    日期: 2011
    上傳時間: 2011-10-27 11:23:49 (UTC+8)
    摘要: 具可分解性與相容性之生物材料,已被廣泛使用在生物醫學上,因其具有良好的分解性以及在生物體內不會造成免疫反應,因此可大幅的降低對生物體的細胞毒性。在本研究中,探討不同分子量之聚乙二醇( Poly ethylene glycol )(PEG)與異氰酸酯( Diisocyanatohexane )(NCO)反應,合成具有胺基甲酸酯(Urathane)之載體主鏈,再將ssDNA銜接上載體,最後因合成的兩個載體上具有相互互補的ssDNA,會因為互補關係而形成高分子聚合物,載體上以螢光物質( 6FAM-ssDNA )為藥物之模擬,最後再將微脂粒(Lipofectamine)將產物包覆形成奈米複合體。分子結構以紅外線光譜儀(FT-IR)及核磁共振儀(NMR)來確定,膠體電泳用以觀察相互互補的ssDNA是否結合上載體,並利用相關儀器及方法測試載體之裂解能力,大小及細胞毒性。
    Biocompatible and degradable biomaterials have been widely used in biomedical applications due to low cytotoxicity and appropriate immune response. Polyurethane was synthesized through the reaction between PEG ( polyethylene glycol ) with different molecular weight and diisocyanatohexane and then grafted single strand DNA. Carrier was finished via the complementary between polyurethane containing single strand DNA ( ss-DNA ) and another single strand DNA ( 6FAM-ssDNA ). Nano-liposomes containing carriers were completed using lipofectamine. Molecular structure of carrier was identified by the fourier transform infrared spectrum ( FT-IR ) and nuclear magnetic resonance spectroscopy ( NMR ). The other characterizations were observed using gel electrophoresis, dynamic light scanning, and cell cytotoxicity.
    關聯: 校內一年後公開,校外永不公開,學年度:99,82頁
    Appears in Collections:[藥學系(所)] 博碩士論文

    Files in This Item:

    File Description SizeFormat

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback