新穎生物可降解性之奈米型藥物載體PEG-dsDNA合成及細胞內釋控之研究

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Title:	新穎生物可降解性之奈米型藥物載體PEG-dsDNA合成及細胞內釋控之研究 Studies on Syntheses of Novel Biodegradable Nanoscale Drug Carriers PEG-dsDNA and Intracellular Drug Delivery
Authors:	楊釗凱
Contributors:	嘉南藥理科技大學：藥物科技研究所蕭明達
Keywords:	聚乙二醇胺基甲酸酯藥物載體異氰酸酯細胞毒性 Urethane Drug vector PEG NCO Cytotoxicity
Date:	2011
Issue Date:	2011-10-27 11:23:49 (UTC+8)
Abstract:	具可分解性與相容性之生物材料，已被廣泛使用在生物醫學上，因其具有良好的分解性以及在生物體內不會造成免疫反應，因此可大幅的降低對生物體的細胞毒性。在本研究中，探討不同分子量之聚乙二醇( Poly ethylene glycol )(PEG)與異氰酸酯( Diisocyanatohexane )(NCO)反應，合成具有胺基甲酸酯(Urathane)之載體主鏈，再將ssDNA銜接上載體，最後因合成的兩個載體上具有相互互補的ssDNA，會因為互補關係而形成高分子聚合物，載體上以螢光物質( 6FAM-ssDNA )為藥物之模擬，最後再將微脂粒(Lipofectamine)將產物包覆形成奈米複合體。分子結構以紅外線光譜儀(FT-IR)及核磁共振儀(NMR)來確定，膠體電泳用以觀察相互互補的ssDNA是否結合上載體，並利用相關儀器及方法測試載體之裂解能力，大小及細胞毒性。 Biocompatible and degradable biomaterials have been widely used in biomedical applications due to low cytotoxicity and appropriate immune response. Polyurethane was synthesized through the reaction between PEG ( polyethylene glycol ) with different molecular weight and diisocyanatohexane and then grafted single strand DNA. Carrier was finished via the complementary between polyurethane containing single strand DNA ( ss-DNA ) and another single strand DNA ( 6FAM-ssDNA ). Nano-liposomes containing carriers were completed using lipofectamine. Molecular structure of carrier was identified by the fourier transform infrared spectrum ( FT-IR ) and nuclear magnetic resonance spectroscopy ( NMR ). The other characterizations were observed using gel electrophoresis, dynamic light scanning, and cell cytotoxicity.
Relation:	校內一年後公開，校外永不公開，學年度：99，82頁
Appears in Collections:	[Dept. of Pharmacy] Dissertations and Theses

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