實驗結果顯示，在不同萃取法萃取龍眼花，以加熱迴流萃取法具有較佳之產率為18.4%，而在總酚含量測定試驗中，以加熱迴流萃取法之龍眼花萃取液之總酚含量為 252.2 ± 0.1 GA mg/extract g 為最高含量。在清除 DPPH自由基之清除率及清除 ABTS＋∙ 自由基清除率中，皆以加熱迴流萃取法較其他兩種方法效果為佳，其 IC50 分別為 20.3 ± 0.2 µg/mL及 2.8 ± 0.8 µg/mL。
raditional Chinese medicine has become a mainstream in the development of active ingredients for the cosmetic industry. In this study, Longan flower were employed to develope cosmetics, this study was divided into five parts. The first part is to extract by different methods, such as refluxing , microwave, immersed, and calculated the extract yield. Secondly, we evaluated the different antioxidant activity of scavenging capacities, and chose the better extract to add in the formulation. Thirdly, we tested the safety of Longan flower extract. To continued, we added the Longan flower extract to the cosmetic formula , and that evaluated the stability and rheological property.Finally, we chose the stability product to evaluated the efficacy, in vivo.
The results showed that, in the different extract methods which is refluxing had the better extract yield of 18.4%.The experiment of total phenolic content, the Longan flower extracts by refluxing had the highest content is 252.2� 0.1 GA mg / extract g. In the DPPH free radical scavenging and the trolox equivalent antioxidant capacity experiment, both of refluxing had the best effect than others, the IC50 value 20.3 � 0.2 µg / mL and 2.8 � 0.2µg / mL, respectively.
We chose the better antioxidant effect of longan flower extract to experiment of safety. The result which in the Cytotoxicity test at 24and 48 hrs showed that, it had the effect of cell activation and non-toxic. In the skin irritation experiment which is smeared on the inside of forearm for up to 24 and 48 hr, the result indicated that will not induce allergic reactions. Total plate count showed that the essential and cream were 104/ mL and 121 /g, respectively. Both are in the qualified range, and also not detected Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.
While in the Longan flower cosmetics of rheological and stability, its Essence and Cream of rheological properties, before and after the accelerate consistency by little change, and in the stability both more than 60 days. Through test after activity and stability, Essence and Cream of Longan flower by human skin test, has the well effect of anti-wrinkle by Essence and Cream, in the future Longan flower will increase cosmetics, development of the effect of anti-wrinkle product.