Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/2380
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    標題: 人類心臟型脂肪酸結合蛋白免疫分析試片及感測系統之開發研究
    Studies on Development of an Immunosensor for Human Heart-type Fatty Acid Binding Protein Based on Surface Plasmon Resonance
    作者: 周淑芬;Shu-Fen Chou
    貢獻者: 生物科技系
    關鍵字: human heart-type fatty acid binding protein(HFABP)
    immunosensor
    surface plasmon resonance (SPR)
    日期: 2005
    上傳時間: 2008-07-31 14:37:39 (UTC+8)
    出版者: 台南縣：嘉南藥理科技大學生物科技系
    摘要: A direct human heart-type fatty acid binding protein (HFABP) immunosensor was
    developed using anti- HFABP monoclonal antibodies (MAbs) immobilized on the gold
    surface of a self-assembled surface plasmon resonance (SPR) apparatus. A kind of
    self-assembled monolayer (SAM) prepared by cystamine-protein Amethod was applied
    to immobilize the MAbs. Ten cycles of measurements could be performed on the same
    chip regenerated with a 0.1 M HCl solution. A linear relationship existed between the
    angle shifts (millidegrees) and the log values of HFABP concentrations in the range
    from 0.1 to 300 ng/mL in buffer and artificial human serum. When used for 15 days,
    the angle shifts were all >95% of those on the response at the first day. A 1 M NaOH
    solution was used for clearing nonspecific binding in artifical human serum. The SPR
    sensor offers advantages of simplicity of immobilization, high sensitivity, high
    specificity, low sample requirement, high reusability, no label and no pretreatment etc.
    關聯: 計畫編號：NSC94-2213-E-041-003
    显示于类别:[生物科技系(所)] 科技部計畫

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