人類心臟型脂肪酸結合蛋白免疫分析試片及感測系統之開發研究

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > MOST Project > Item 310902800/2380

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Title:	人類心臟型脂肪酸結合蛋白免疫分析試片及感測系統之開發研究 Studies on Development of an Immunosensor for Human Heart-type Fatty Acid Binding Protein Based on Surface Plasmon Resonance
Authors:	周淑芬;Shu-Fen Chou
Contributors:	生物科技系
Keywords:	human heart-type fatty acid binding protein(HFABP) immunosensor surface plasmon resonance (SPR)
Date:	2005
Issue Date:	2008-07-31 14:37:39 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學生物科技系
Abstract:	A direct human heart-type fatty acid binding protein (HFABP) immunosensor was developed using anti- HFABP monoclonal antibodies (MAbs) immobilized on the gold surface of a self-assembled surface plasmon resonance (SPR) apparatus. A kind of self-assembled monolayer (SAM) prepared by cystamine-protein Amethod was applied to immobilize the MAbs. Ten cycles of measurements could be performed on the same chip regenerated with a 0.1 M HCl solution. A linear relationship existed between the angle shifts (millidegrees) and the log values of HFABP concentrations in the range from 0.1 to 300 ng/mL in buffer and artificial human serum. When used for 15 days, the angle shifts were all >95% of those on the response at the first day. A 1 M NaOH solution was used for clearing nonspecific binding in artifical human serum. The SPR sensor offers advantages of simplicity of immobilization, high sensitivity, high specificity, low sample requirement, high reusability, no label and no pretreatment etc.
Relation:	計畫編號：NSC94-2213-E-041-003
Appears in Collections:	[Dept. of Biotechnology (including master's program)] MOST Project

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