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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/23480

    標題: Nucleotide sequence and expression of the isoamylase gene from an isoamylase-hyperproducing mutant,Pseudomonas amyloderamosa JD210
    作者: Jiau H. Chen
    Zuei Y. Chen
    The Y. Chow
    Jinn C. Chen
    Shieh T. Tanand
    Wen H. Hsu
    貢獻者: 食品系
    關鍵字: Isoamylase gene
    Nucleotide sequence
    Gene expression
    DNA sequence
    P. amyloderamosa
    日期: 1991
    上傳時間: 2011-01-04 16:45:01 (UTC+8)
    摘要: The isoamylase gene (ISO) of Pseudomonas amyloderamosa JD210, an isoamylase-hy roducin mutan was cloned in an isoamylase-deficient and transformable mutant strain K3l. By deletivn analysis, the ISO gene was found to be located within a 3.3 kilvbases Bamfll fragment. Ils nuclootide sequence contained an open reading franle of 2328 nucleotides (776 amino acids) encoding a secreted isvamylase precursor. TI1e ISO gene fragment was inserted into plasmids pKT230 and pBR 322 in opposite orientations. The expression of the ISO gene in the constructed plasntids was compared in P. _amyloderamosa K31, Pseudonzonas aeruginosa PAOI-161, Pseudomonas pufida mt-2 and Escherichia cali HBIOI. In all transfvrmed cells, the niajority of the isoamylase Qlduc_ed was secreted and hieher isoainylase activities were obtained in transformants with the transcriptional direction ul the IDU gene similu to the nearby drug-determinant gene of the vector.
    關聯: 嘉南學報 17期 : p.A1-A7 轉載 Biochimica et Biophysica Acta, 1087(1990) p.309-315
    Appears in Collections:[嘉南學報] 17期 (1991)
    [ 食品科技系 ] 期刊論文

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